Pyrocatechol Alleviates Cisplatin-Induced Acute Kidney Injury by Inhibiting ROS Production

Figure 4

Pyrocatechol alleviates cisplatin-induced apoptosis through P38/JNK pathway. (a) Western blotting was used to measure the expression of MAPK pathway proteins after treatment with cisplatin and pyrocatechol. The expression of MAPK pathway proteins, including p38, p-p38, c-Jun N-terminal kinase (JNK), p-c-Jun N-terminal kinase (p-JNK), extracellular signal-regulated kinase (ERK), and p extracellular signal-regulated kinase (p-ERK). (b) Western blotting was used to measure the expression of MAPK pathway proteins after treatment with cisplatin and ROS inhibitor (NAC, N-Acetyl-L-cysteine). (c) Western blotting was used to measure the expression of proteins treated with the P38/JNK agonist anisomycin, including p38, p-p38, JNK, p-JNK, and Cleaved Caspase-3. (d) Flow cytometric analysis of apoptosis in anisomycin-treated HK-2 cells. Values are presented as the (). The statistical analysis was performed with two-way ANOVA.

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