Pyrocatechol Alleviates Cisplatin-Induced Acute Kidney Injury by Inhibiting ROS Production

Figure 5

The antiapoptotic effect of pyrocatechol is dependent on glutathione peroxidase 4 (GPX4). (a) The knockout efficacy was investigated using western blotting. (b) GPX4-KO HK-2 cells were treated with cisplatin (20 μM) in the absence or presence of pyrocatechol for 24 hours, and cell viability was then determined. (c) GPX4-KO HK-2 cells were treated with cisplatin (20 μM) in the absence or presence of pyrocatechol for 24 hours, and then, their ROS levels were measured by flow cytometry. Values are presented as the (). The statistical analysis was performed with two-way ANOVA.

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