Four-Octyl Itaconate Protects Chondrocytes against H<sub>2</sub>O<sub>2</sub>-Induced Oxidative Injury and Attenuates Osteoarthritis Progression by Activating Nrf2 Signaling

<div>Keap1 knockout abolishes OI-induced cytoprotection from H<sub>2</sub>O<sub>2</sub>. Stable chondrocytes with the CRISPR/Cas9-Keap1-KO-GFP construct (ko-Keap1) or the CRISPR/Cas9 control vector (“Cas9-C”) were established, and the expression of the listed genes was tested (a, b). Following treatment with OI (25 <i>μ</i>M), chondrocytes were added by H<sub>2</sub>O<sub>2</sub> (300 <i>μ</i>M). Cell viability and apoptosis were assessed using CCK-8 (c) and Trypan blue assays (d), respectively. 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Oxidative Medicine and Cellular Longevity

fig5

Figure 5

Figure 5: Four-Octyl Itaconate Protects Chondrocytes against H<sub>2</sub>O<sub>2</sub>-Induced Oxidative Injury and Attenuates Osteoarthritis Progression by Activating Nrf2 Signaling 

Figure 5 | Four-Octyl Itaconate Protects Chondrocytes against H2O2-Induced Oxidative Injury and Attenuates Osteoarthritis Progression by Activating Nrf2 Signaling 