Energy-Stress-Mediated AMPK Activation Promotes GPX4-Dependent Ferroptosis through the JAK2/STAT3/P53 Axis in Renal Cancer

Figure 2

Energy stress promotes ferroptotic cell death through AMPK activation. (a and b) Western blotting assay verifies the activation of AMPK after 0-Glu treatment and the knockdown efficacy of two siAMPK. (c) Cell death measurement shows the alleviation of cell death by Com C, siAMPK, and Fer-1 after 0-Glu and erastin treatment. (d) TEM images shows that Com C, siAMPK, and Fer-1 treatments recover mitochondrial morphology. Black arrows indicate mitochondria. Scale bars: 1 μm. Independent experiments were repeated three times, and representative data were shown. (e–g) Com C, siAMPK, and Fer-1 treatments reverse the changes of GSH, MDA, and cellular iron level caused by 0-Glu and erastin treatment. Data shown represent from at least three independent experiments. Comparisons were performed using Student’s -test and one-way ANOVA. NC: negative control; Com C: Compound C; Fer-1: ferrostatin-1. and .

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