DJ-1/FGFR-1 Signaling Pathway Contributes to Sorafenib Resistance in Hepatocellular Carcinoma

Figure 2

DJ-1 induced of angiogenesis and migration in ECDHCC-1 cells. (a) DJ-1 secretion in HCC cells. HepG2 and HUH-7 cells were seeded for culture medium (CM) collection. The CM was harvested and examined by ELISA for DJ-1 expression after 24 and 48 h. (b) Purification of DJ-1 as indicated in the elution lane. (c and d) Product verification of DJ-1 by western blotting analysis with anti-His tag (c) and anti-DJ-1 (d) specific antibody was performed, respectively. (e–g) Fluorescence microscopy imaging of capillary tube formation (e) and quantification of DJ-1 induced tube nodes (f) and tube length (g) using tube formation assay were conducted. (h and i) Wound healing image (h) and quantification of the relative scratch width (i) of ECDHCC-1 cells induced by DJ-1 were analyzed. Con: Control group. and versus Con. (original magnifications: for fluorescence).

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