DJ-1/FGFR-1 Signaling Pathway Contributes to Sorafenib Resistance in Hepatocellular Carcinoma

Figure 6

Downregulation of FGFR1 affected the apoptosis of sorafenib-resistant HCC cells. (a) Cell viability of THLE-2, HepG2, and HUH-7 after treatment with indicated concentration of sorafenib was assessed by CCK8 assay, and half-maximal inhibitory concentration (IC₅₀) was calculated with a linear fit. (b) Sorafenib-resistant HUH-7 cell line (HUH-7/R) was constructed, and the cell viability was assessed to define the resistance index (RI) of the HUH-7/R cell line. (c) Western blotting analysis of multidrug resistance marker of P-gp and MRP1 was detected. (d and e) Flow cytometry assessment of cell apoptosis by annexin-V/PI (d) and two representative groups after transient knockdown of FGFR1 in HUH-7 and HUH-7/R cells was statistically analyzed (e). (f)–(l) Western blotting analysis of p-FGFR1/FGFR1, cleaved caspase 3, cleaved caspase 9, Bax, and Bcl-2 was carried out in HUH-7 and HUH-7/R cells after transient knockdown of FGFR1 and combined treatment with sorafenib and DJ-1. NS: Not significant, versus DJ-1 group, and versus the vehicle or DJ-1 alone.

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