Hinokitiol Protects Cardiomyocyte from Oxidative Damage by Inhibiting GSK3β-Mediated Autophagy

Figure 2

Hinokitiol prevented AC16 cells from H2O2-induced apoptosis. (a) Cells were treated as indicated and straining with Annexin V/7AAD; the apoptosis rate was detected by flow cytometry. (b) Quantification of the percentage of apoptotic rate in (a), ns; , , , . (c) Western blotting analysis of AC16 cells treated as indicated. Quantitative analysis of each bands was represented in numerical form under images, respectively. (d) Representative images of AC16 cells after straining with Hochest33258, the yellow arrow indicated apoptotic cells. (e) Quantification of the percentage of apoptotic cell in (d), ns; , , .

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