circPTP4A2-miR-330-5p-PDK2 Signaling Facilitates In Vivo Survival of HuMSCs on SF-SIS Scaffolds and Improves the Repair of Damaged Endometrium

Figure 3

The expression of circPTP4A2 is significantly elevated in HuMSCs cultured on SF-SIS scaffolds. (a) The profile of circRNA from normal HuMSCs and HuMSCs cultured on the SF-SIS scaffolds was analyzed by RNA-seq of ribosomal RNA-depleted total RNA. (b) The differential expression of total circRNAs was directly revealed by volcanic eruption-type clustering analysis. (c) Several significantly upregulated circRNAs (circUXSI, circPTP4A2, circCNTRL, circEPSTM, circSFMBT2, circZNF680, and circEMB) were verified via real-time PCR. (d) The expression level of circPTP4A2 in the different animal models was analyzed via real-time PCR. (e) CircPTP4A2 was identified using real-time PCR, indicating resistance between circPTP4A2 and RNase R. RNase R treatment significantly reduced PTP4A2 mRNA. (f) Subfractional real-time PCR and (g) fluorescence in situ hybridization (FISH) assay were used to analyze the subcellular location of circPTP4A2 in HuMSCs. , relative to the indicated group.

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