miR-340-5p Alleviates Oxidative Stress Injury by Targeting MyD88 in Sepsis-Induced Cardiomyopathy

Figure 5

The effect of miR-340-5p overexpression or knockdown on the MyD88 level and the interaction of miR-340-5p with MyD88 mRNA. (a) Western blotting analysis of MyD88 expression in the mimic-NC, mimic-NC+LPS, or miR-340-5p-mimic+LPS group. (b) Western blotting analysis of the MyD88 level inhibitor-NC, inhibitor-NC+LPS, or miR-340-5p-inhibitor+LPS group. (c) The predicted binding sites of miR-340-5p with the 3UTR of MyD88 in different species. (d, e) The dual-luciferase reporter activities of MyD88-mut 3UTR (d) or MyD88-wt 3UTR (e) in blank, mimic-NC, or miR-340-5p group. (f) Detection of MyD88 enrichment on Bio-NC or Bio-miR-340-5p in HL-1 cells via RNA pull-down assay. ; versus mimic-NC (a), inhibitor-NC (b), or Bio-NC group (f); ^# versus mimic-NC+LPS (a), inhibitor-NC+LPS (b), or mimic-NC group (e). n.s.: no significant; mut: mutant; wt: wild type.

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