Block of Proliferation 1 Promotes Proliferation, Invasion and Epithelial Mesenchymal Transformation in Gastric Cancer

Figure 3

BOP1 promotes proliferation and inhibits apoptosis in GC cells. The expression of BOP1 mRNA (a) and protein (b) were measured in 5 different gastric cell lines including GSE-1, HGC-27, N87, MGC803 and BCG823. The expression of BOP1 mRNA (c) and protein (d) were measured in BOP1-overexpressed N87 cell and BOP1-silencing HGC-27 cell. (e) Cell viability was detected by CCK-8 in different time points (0, 24, 48, 72 and 96 hours after transfection) in HGC-27 and N87 cells. (f) The proportion of apoptotic cells were detected by flowcytometry. (g) The expression level of caspase 3 and p21 were measured. The data are expressed as the means ± s.e.m. of three experiments. (P < 0.05, P < 0.01, P < 0.001).

(a)

(b)

(c)

(d)

(e)

(f)

(g)