Mulberrin Confers Protection against Doxorubicin-Induced Cardiotoxicity via Regulating AKT Signaling Pathways in Mice

Figure 7

Infection of AKT-dominant negative mutant blocked the protection of mulberrin against DOX-induced cardiac injury in vivo. The phosphorylation of AKT (a) was detected via western blotting in mice at 4 weeks after AAV9-dnAKT or AAV9-Con injection. Heart weight (b) and myocardial LDH (c) were detected to evaluate DOX-induced cardiac injury. Cardiac function including ejection fraction (d) and cardiac output (e) were detected using an invasive hemodynamic monitoring. Oxidative stress markers, 3-NT (f) and protein carbonyl content (g), were quantified by the commercial kits. Nrf2 protein expression (h, i) was detected via western blotting. The nuclear accumulation of NF-κB protein (h, i) was detected in an isolated nuclear fraction. The levels of inflammatory factors (j, k) were also quantified. Myocardial apoptosis was evaluated by caspase 3 activity (l) and DNA fragmentation test (m). Data are presented as . for each group. compared with the control groups.

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