Theaflavin-3,3-Digallate Protects Cartilage from Degradation by Modulating Inflammation and Antioxidant Pathways

Figure 3

TFDG protects the cartilage matrix of chondrocytes. (a and b) The gene levels of Mmp3, Mmp13, and Adamts5 and Col2a1, Sox9, and ACAN were analyzed by RT-qPCR. (c–j) The protein expressions of COL2, SOX9, aggrecan, MMP13, MMP3, and ADAMTS5 were analyzed by western blotting. The grey values normalized with β-actin and control group were quantified by ImageJ. The bar graph shows the of data (). , , , and . (k and l) COL2 and MMP13 protein expressions were detected by immunofluorescence. Scale bar: 100 μm.

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