Theaflavin-3,3-Digallate Protects Cartilage from Degradation by Modulating Inflammation and Antioxidant Pathways

Figure 4

TFDG reduces IL-1β-induced ROS accumulation by activating the Nrf2 pathway. (a and b) The intensity mean of ROS marked by DCFH-DA was quantified by flow cytometry. (c–h) The protein expressions of Nrf2 in nucleus and cytoplasm, HO-1, and SOD2 were analyzed by western blotting. The grey values of Nrf2 protein in nucleus normalized with Lamin B and other protein normalized with β-actin and control group were quantified by ImageJ. The bar graph shows the of data (). , , , and . (i) Nrf2 protein expressions were detected by immunofluorescence. Scale bar: 100 μm.

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