Theaflavin-3,3-Digallate Protects Cartilage from Degradation by Modulating Inflammation and Antioxidant Pathways

Figure 5

The blockade of Nrf2/HO-1 signaling partly reverses the protective effect of TFDG. (a and b) The intensity mean of ROS marked by DCFH-DA was quantified by flow cytometry. (c–n) The protein expressions of Nrf2 in nucleus, HO-1, SOD2, COL2, SOX9, aggrecan, MMP13, MMP3, and ADAMTS5 were analyzed by western blotting. The grey values of Nrf2 protein in nucleus normalized with Lamin B and other protein normalized with β-actin and control group were quantified by ImageJ. The bar graph shows the of data (). , , , and .

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