[Retracted] Human Umbilical Cord Mesenchymal Stem Cell-Derived Exosome Repairs Endometrial Epithelial Cells Injury Induced by Hypoxia via Regulating miR-663a/CDKN2A Axis

Figure 5

HucMSC-Ex increased miR-663a in hypoxia-treated EECs, and miR-663a negatively regulated CDKN2A expression. (a) DEmiRNA in the hypoxia, normal, and hypoxia+hucMSC-Ex groups was visualized by Volcano plot. Red and blue dots showed expressions increased and decreased miRNAs, respectively. (b) Profiling of miRNAs in the normal, hypoxia, and hypoxia+hucMSC-Ex groups was displayed by heat map. Blue color indicated relative low level and red color indicated relative high level in comparison with the normal group (left panel). (c) The miR-663a level in EECs was detected by qRT-PCR. (d) The target sequence of miR-663a and CDKN2A was predicted by online website TargentScanHuman (https://www.targetscan.org/vert_80/). (e) qRT-PCR was carried out for detecting the mRNA levels of CDKN2A in EECs. (f) The miR-663a level in hypoxia-induced EECs transfected by miR-663a mimics was detected by qRT-PCR. (g–l) The protein expression of CDKN2A (p16INK4a), E-cadherin, N-cadherin, Bax, and Bcl-2 in hypoxia-induced EECs transfected with miR-663a mimics or NC was detected using western blot. , . Data are expressed as . .

(a)

(b)

(c)

(d)

(e)

(f)

(g)

(h)

(i)

(j)

(k)

(l)