Research Article
Tanreqing Injection Regulates Cell Function of Hypoxia-Induced Human Pulmonary Artery Smooth Muscle Cells (HPASMCs) through TRPC1/CX3CL1 Signaling Pathway
Figure 3
Human pulmonary artery smooth muscle cells (HPASMCs) were treated using 0.01 crude drug g/ml TRQ, 50 μM SKF96365, 60 nM cysteine, and/or 200 ng/ml CX3CL1 and undergoing hypoxia (2% O2) for 24 h. (a) ROS of cells was assayed using the Reactive Oxygen Species Assay Kit. Red fluorescence indicated ROS. (b) Calcium (Ca2+) changes were detected using calcium fluorescent probe Fluo-3 AM. Green fluorescence indicated Ca2+. (c) Relative ROS (to normal group) was analyzed. and vs. normal group. # and ## vs. hypoxia group. . (d) The ability to inhibit hydroxyl radicals of cells was determined. and vs. normal group. # and ## vs. hypoxia group. . (e) Ca2+ was analyzed (to normal group). and vs. normal group. # and ## vs. hypoxia group. .
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