[Retracted] Siglec-15 Regulates the Inflammatory Response and Polarization of Tumor-Associated Macrophages in Pancreatic Cancer by Inhibiting the cGAS-STING Signaling Pathway

Figure 2

Deletion of Siglec-15 inhibits the polarization of macrophages towards M2. (a–c) Bone marrow cells from wild-type and Siglec-15 knockout mice were extracted in vitro and induced into bone marrow-derived macrophages (BMDMs). 100 ng/mL LPS and 20 ng/mL IFN-γ to stimulate for 12 hours; induce them into M1-type macrophages and detect the inflammatory factors expressed by M1-type macrophages by qPCR. (d–f) 20 ng/mL IL-4 to stimulate BMDMs cells for 24 h or 48 h to induce into M2-type macrophages. qPCR detects the marker genes expressed by M2-type macrophages. (g–i) Tumor supernatant conditioned medium to stimulate BMDM cells for 24 h or 48 h to induce into M2-type macrophages, and qPCR to detect the marker genes expressed by M2-type macrophages. The experiments were repeated three times, and each experiment was triplicated.

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