[Retracted] Siglec-15 Regulates the Inflammatory Response and Polarization of Tumor-Associated Macrophages in Pancreatic Cancer by Inhibiting the cGAS-STING Signaling Pathway

Figure 4

Siglec-15 interacts with Glut1 to regulate macrophage polarization. (a) Immunoblotting of cell lysate co-transformed with Siglec-15-FLAG+Glut1-HA with FLAG antibody immunoprecipitation. (b) GST protein pulldown results of GST/GST-Glut1 protein and Glut1 protein. (c) qPCR assay indicated that knocking down Glut1 can overexpress Siglec-15-induced upregulation of Arg1 expression which was a biomarker of M2 phenotype macrophage. (d) Western blotting assay indicated that knocking down Glut1 can overexpress Siglec-15-induced upregulation of Arg1 expression which was a biomarker of M2 phenotype macrophage. (e) qPCR assay indicates that knocking down Glut1 can overexpress Siglec-15-induced upregulation of iNOS which is another biomarker of M2 phenotype macrophage. (f) Western blotting assay indicate that knocking down Glut1 can overexpress Siglec-15-induced upregulation of iNOS which is another biomarker of M2 phenotype macrophage. The experiments were repeated three times, and each experiment was triplicated.

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