[Retracted] Siglec-15 Regulates the Inflammatory Response and Polarization of Tumor-Associated Macrophages in Pancreatic Cancer by Inhibiting the cGAS-STING Signaling Pathway

Figure 5

Siglec-15 regulates macrophage inflammatory factors partly dependent on STING. (a) BMDMs of bone marrow-derived macrophages from WT and Siglec-15 KO mice were extracted, and 50 ng/mL IL-4 stimulated the cells. The proteins were collected according to different experimental time points, and the cGAS-STING signaling pathway was detected by western blot. (b) BMDMs of bone marrow-derived macrophages from WT and Siglec-15 KO mice were extracted, and conditioned medium containing Pan-02 supernatant stimulated the cells. The proteins were collected according to different experimental time points, and the cGAS-STING signaling pathway was detected by western blot. (c) After extracting Sigle-15KO mouse peritoneal primary macrophages, stimulated with cisplatin for 24 hours, the protein expression of the cGAS-STING signaling pathway conditioned medium containing Pan-02 supernatant was as shown in the figure. (d) After extracting Sigle-15KO or Sigle-15 OE mouse peritoneal primary macrophages, after IL-4 stimulation, the expression of cGAS-STING signaling pathway molecule protein-conditioned medium containing Pan-02 supernatant was as shown. The experiments were repeated three times, and each experiment was triplicated.

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