Mammalian Sterile 20-Like Kinase 1 Mediates Neuropathic Pain Associated with Its Effects on Regulating Mitophagy in Schwann Cells

Figure 4

Mst1 knockdown promoted Parkin translocation to mitochondria in cultured SCs. (a) Protein expression of Mst1, Parkin, and LC3-II with GAPDH as a loading control and relative level of LC3-II/I ratio. ; . Statistical significance was determined by Student’s -test. Blank group: cells were cultured in DMEM with 10% FBS for 24-h. Starvation group: cells were cultured in DMEM with 1% FBS for 24-h. (b) Protein expression of p62, Mst1, Parkin, Tom20, and LC3-II with GAPDH as a loading control and relative level of LC3-II/I ratio. ; . Statistical significance was determined by one-way ANOVA, followed by Bonferroni’s post hoc test. (c) Protein expression of cyto-p62, mito-p62, cyto-Parkin, and mito-Parkin, with GAPDH as a loading control for proteins in the cytosol and COX-IV as a loading control for mitochondrial proteins. ; . Statistical significance was determined by one-way ANOVA, followed by Bonferroni’s post hoc test. All data were expressed as the . Control group: no treatment. si-NC group: cells were treated with scrambled si-RNA as negative control. si-Mst1 group: cells were treated with Mst1 siRNA. 24 h after transfection, all groups were cultured in DMEM with 1% FBS for another 24 h.

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