Mammalian Sterile 20-Like Kinase 1 Mediates Neuropathic Pain Associated with Its Effects on Regulating Mitophagy in Schwann Cells

Figure 5

Mst1 knockdown sustained mitochondrial function and prevented mitochondria apoptosis. (a) Measurement and quantitative analysis of mean fluorescence intensity for DCFH-DA staining. ; . Statistical significance was determined by Student’s -test. (b) Measurement and quantitative analysis of JC-1 staining, expressed as ratio of green fluorescence intensity to red fluorescence intensity. . Statistical significance was determined by Student’s -test. si-NC group: cells were treated with scrambled si-RNA as negative control. si-Mst1 group: cells were treated with siRNA of Mst1. 24 h after transfection, all groups were cultured in DMEM with 1% FBS for another 24 h. (c) Protein expression of Caspase9, Bax, and Cyt-c, with GAPDH as loading control. ; . Control group: no treatment. si-NC group: cells were treated with scrambled si-RNA as negative control. si-Mst1 group: cells were treated with siRNA of Mst1. 24 h after transfection, all groups were cultured in DMEM with 1% FBS for another 24 h. Statistical significance was determined by one-way ANOVA, followed by Bonferroni’s post hoc test. All data were expressed as the .

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