circHtra1/miR-3960/GRB10 Axis Promotes Neuronal Loss and Immune Deficiency in Traumatic Brain Injury

Figure 2

Effects of circHtra1 on neuronal proliferation. (a) Expression of circHtra1 in si-circ was verified. (b) After primary cortical neurons were transfected with si-NC or si-circHtra1, a CCK-8 assay was applied to assess cell proliferation. (c) Effect of si-circHtra1 on neuronal maturation (green: MAP2; red: β-tubulin, merged with DAPI). (d, e) Quantification of mean densities of MAP2 (, ) and β-tubulin (, ). Scale bar represents 200 μm. (compared to the si-NC group); effects of circHtra1 on neuronal proliferation. (f) Verification of circHtra1 overexpression. (g) Primary cortical neurons were transfected with NC or circHtra1. (h) Effect of circHtra1 on neuronal proliferation (green: MAP2; red: β-tubulin, merged with DAPI). (i, j) Quantification of mean densities of MAP2 (, ) and β-tubulin (, ). Scale bar represents 200 μm. (compared to the NC group).

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