[Retracted] Antiviral Activity of Plantago asiatica Polysaccharide against Pseudorabies Virus In Vitro

Figure 7

PLP reduces intracellular ROS levels. (a, b) We pretreated PK15 cells by PLP at diverse doses for a 1 h period prior to infection by PRV XJ5 (), followed by PLP treatment for a 24 h period at diverse doses. Flow cytometry was conducted to measure the total fluorescence intensity of more than 10,000 cells in each sample. (c) We pretreated PK15 cells with different concentrations of NAC (2, 4, 8, or 10 mM). Next, we inoculated PRV () in cells for a 1 h period, followed by incubation using the relevant NAC contents within 2% DMEM. (d) PRV XJ5 () was incubated with PLP (100, 200, 400, and 600 μg/mL) for a 1 h period at 37°C; then, we inoculated the pretreated virus to Vero cell for measuring virus titer through TCID₅₀. The error bars indicated the standard deviation from three independent experiments. .

(a)

(b)

(c)

(d)