The E3 Ubiquitin Ligase SYVN1 Plays an Antiapoptotic Role in Polycystic Ovary Syndrome by Regulating Mitochondrial Fission

Figure 1

Downregulated SYVN1 is potentially associated with massive apoptosis in granulosa cells of PCOS patients. (a) The mRNA expression of SYVN1 was detected by RT-qPCR (). , two-tailed unpaired Student’s -test. , , and . (b) The protein levels of SYVN1 were detected by western blotting (). , two-tailed unpaired Student’s -test. , , and . (c) The expression of SYVN1 was detected by immunofluorescence. Red staining indicates SYVN1, and blue staining indicates the nucleus (DAPI).  μm. (d) Granulosa cell apoptosis was detected by TUNEL.  μm. (e) Protein expression of cleaved-caspase-3, Bax, and Bcl-2 of ovarian granulosa cells in each group was detected by western blotting. (control: patients undergoing fertility treatment due to male infertility or tubal infertility disorders; PCOS, patients with polycystic ovary syndrome). The data are expressed as . () , , and . Two-tailed unpaired Student’s -test. Cleaved-caspase-3: , , and ; Bax: , , and ; Bcl-2: , , and .

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