The E3 Ubiquitin Ligase SYVN1 Plays an Antiapoptotic Role in Polycystic Ovary Syndrome by Regulating Mitochondrial Fission

Figure 2

Overexpression of SYVN1 inhibited apoptosis in KGN cells. KGN cells were transfected with empty, SYVN1, control siRNA, or SYVN1 siRNA plasmids for 24 h. (a, b) RT-qPCR and western blot analysis for SYVN1 to test the transfection efficiency for SYVN1 overexpression or inhibition. , , and . One-way ANOVA with Tukey’s multiple comparison test. (a) , . , ; (b) , . , . (c) Apoptosis of KGN cells in each group was detected by flow cytometry. Cartogram of apoptosis of KGN cells in each group. and . One-way ANOVA with Tukey’s multiple comparison test. , . , . (d) Apoptosis of KGN cells in each group was measured by TUNEL staining.  μm. (e) Protein expression of cleaved-caspase-3, Bax, and Bcl-2 in ovarian granulosa cells from each group was detected by western blotting. The data are expressed as . , , and . One-way ANOVA with Tukey’s multiple comparison test. Cleaved-caspase-3: , . , ; Bax: , . , ; Bcl-2: , . , .

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