The E3 Ubiquitin Ligase SYVN1 Plays an Antiapoptotic Role in Polycystic Ovary Syndrome by Regulating Mitochondrial Fission

Figure 5

SYVN1 inhibited apoptosis and mitochondrial fission by promoting Drp1 degradation in KGN cells. KGN cells were transfected with control siRNA, SYVN1 siRNA, Drp1 siRNA, or SYVN1 siRNA+Drp1 siRNA for 24 h. (a) Apoptosis of KGN cells in each group was detected by flow cytometry. Cartogram of cell apoptosis in each group. and . One-way ANOVA with Tukey’s multiple comparison test. , . , , . (b) Apoptosis in each group of KGN cells was measured by TUNEL staining. Scale bars are 50 μm. The percentages of TUNEL-positive cells are shown. (c) Protein expression of cleaved-caspase-3, Bax, and Bcl-2 of ovarian granulosa cells in each group. , , and . One-way ANOVA with Tukey’s multiple comparison test. Cleaved caspase-3: , . , , ; Bax: , . , , ; Bcl-2: , . , , . (d) Imaging mitochondrial morphology in each group by confocal microscopy and MitoTracker Red. Scale bars are 10 μm. Changes in mitochondrial morphology were quantified by the ImageJ software. The data are expressed as .

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