The E3 Ubiquitin Ligase SYVN1 Plays an Antiapoptotic Role in Polycystic Ovary Syndrome by Regulating Mitochondrial Fission

Figure 6

SYVN1 inhibited apoptosis and mitochondrial fission in granulosa cells in rats with polycystic ovary syndrome. Female Sprague Dawley rats were injected subcutaneously with DHEA for 21 days and injected intraperitoneally with saline or SYVN1 inhibitor (LS-102) for 28 days. (a) Ovarian histological staining was performed in each group using H&E. PF: primordial follicles; PrF: primary follicles; SF: secondary follicles; AnF: antral follicles; GF: Graafian follicles; AF: atretic follicles; CF: cystic follicle cysts. (b) Average number of primordial, primary, secondary, antral, Graafian follicles, atretic follicles, and cystic follicles in control, PCOS, and LS-102-treated rats. , , and . One-way ANOVA with Tukey’s multiple comparison test. Primordial follicles: , . Primary follicles: , . ; secondary follicles: , . ; antral follicles: , . ; Graafian follicles: , . ; atretic follicles: , . ; cystic follicles: , . , . (c) The protein levels of SYVN1 and Drp1 were detected in each group by western blotting. and . One-way ANOVA with Tukey’s multiple comparison test. SYVN1: , . , ; Drp1: , . , , . (d) Representative transmission electron microscopy images of the mitochondrial network in ovarian tissues from each group. Asterisks, arrows, and triangles indicate elongated, intermediate (mid), and fragmented mitochondria, respectively. N: nucleus. Scale bar: 2 mm. (e) Protein expression of cleaved-caspase-3, Bax, and Bcl-2 of ovarian granulosa cells in each group was detected by western blotting. The data are expressed as . , , and . One-way ANOVA with Tukey’s multiple comparison test. Cleaved caspase-3: , . , , ; Bax: , . , , ; Bcl-2: , . , , (control group: untreated rats; PCOS group: saline was injected through the abdominal cavity of the PCOS model rats. LS-102 group: LS-102 was injected through the abdominal cavity of the PCOS model rats).

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