Endothelial MicroRNA-483-3p Is Hypertension-Protective

Figure 3

miR-483-3p alleviates Ang II-induced endothelial dysfunction. (a, b) HUVECs were incubated with various concentrations of (a) Ang II (0, 100, 200, 500, and 1,000 nM) for 24 hours or (b) Ang II at 200 nM concentration for different times (0, 12, 24, 36, and 48 hours). miR-483-3p levels in HUVECs were measured by the qPCR analysis. U6 was used as an internal control. (c, d) HUVECs were incubated with Ang II (200 nM) for 24 hours, together with miR-483 mimic (20 nM). (c) The qPCR analysis of mRNA expression of ECs dysfunction-related genes (ACE1, ET-1, TGF-β, and CTGF). (d) Western blotting analysis of these ECs dysfunction-related genes. Data are shown as . Significance for data was carried out by the one-way ANOVA test. ; ; .

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