Endothelial MicroRNA-483-3p Is Hypertension-Protective

Figure 5

ARBs induce miR-483-3p expression in hypertension subjects and spontaneously hypertensive rats. (a) HUVECs were treated with candesartan (Candi, 2 μM/L), telmisartan (Telmi, 5 μM/L), losartan (Los, 5 μM/L), and valsartan (Val, 5 μM/L) for 24 hours, which was followed by qPCR analysis for miR-483-3p level. U6 was an internal control. (b–e) Spontaneously hypertensive rats (SHR) were administered intragastrically with saline or telmisartan (5 mg/kg/day) for 8 weeks. Homologous Wistar-Kyoto (WKY) rats were considered the control group. (b) Systolic blood pressure (SBP). (c) Relative mRNA expression of ACE1, ET-1, TGF-β, and CTGF in the arteries of SHR. (d) Serum and (e) aortic miR-483-3p levels in SHR treatment with telmisartan. (f) Serum miR-483-3p levels in hypertension patients who orally took telmisartan (80 mg/day) before or after 3 months. Data are indicated as . The one-way ANOVA test was used for statistical analysis. ; ; .

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