KCNH6 Enhanced Hepatic Glucose Metabolism through Mitochondrial Ca²⁺ Regulation and Oxidative Stress Inhibition

Figure 2

KCNH6 affected liver glucose metabolism in HepG2 cells. (a) Measurement of the G6pase, PEPCK, IRS2, and Glut2 levels in HepG2 cells expressing Kcnh6 as determined by qRT-PCR (). (b) Western blot results showing the G6pase, PEPCK, IRS2, and Glut2 levels in HepG2 cells overexpressing KCNH6 (). Glucose uptake measurements were performed with flow cytometry at (c) the basal level () and (d) after insulin stimulation () in HepG2 cells overexpressing KCNH6. (e) Study of glycogen synthesis in HepG2 cells overexpressing KCNH6 using a glycogen synthesis assay kit (). , , and vs. the Ctrl group. ^#, ^##, and ^### vs. the Kcnh6 group. Statistical comparisons were calculated using the Mann–Whitney test.

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