KCNH6 Enhanced Hepatic Glucose Metabolism through Mitochondrial Ca²⁺ Regulation and Oxidative Stress Inhibition

Figure 3

KCNH6 attenuated intracellular and mitochondrial calcium levels in primary hepatocytes. (a) Detection of KCNH6 expression in the mitochondria (). (b) Calcium ion levels in mouse liver mitochondria as determined by Rhod-2-AM staining ( for the WT group; for the KO group). (c) Fluo-4 AM calcium orange staining was performed for detecting changes in calcium ion levels in the cytoplasm of primary liver cells in mice ( for the WT group and the KO group). and vs. the WT group. Statistical comparisons were calculated using the (a, c) Mann–Whitney test and (b) unpaired-sample -test.

(a)

(b)

(c)