In Vitro Synergistic Inhibitory Activity of Natural Alkaloid Berberine Combined with Azithromycin against Alginate Production by Pseudomonas aeruginosa PAO1

Table 1

Sequences of primers used for RT-qPCR.

Gene	Type	Primer sequence(5-3)	Tm (°C)	Length (bp)
algG	Forward; Reverse	GCTGGTACGGCTTCTATTGCTACG; ATCATCTTCTCCACGCCTAC	60.2; 61.4	24; 20
algD	Forward; Reverse	AGAAGTCCGAACGCCACA; TCCAGCTCGCGGTAGAT	56.8; 56.0	18; 17
algR	Forward; Reverse	AGACCGGCTACGGCTACA; GCGTCGTGCTTCTTCAGTT	58.8; 55.9	18; 19
ropB	Forward; Reverse	AGGCCGTGAGCAGGGAT; GGTGGTGCGACCGATGT	60.1; 58.9	17; 17