TREM-1 Modulates Dendritic Cells Maturation and Dendritic Cell-Mediated T-Cell Activation Induced by ox-LDL

Figure 3

TREM-1 plays an important role in DCs maturation and subsequent T-cell proliferation induced by ox-LDL. (a) The process of monocytes differentiated into immature DCs (imDC), at 0, 1, 2, 3, 4, 5, and 6 days; the expressions of CD14 were determined by flow cytometry. (b) At day 6, immature DCs were treated with LDL or ox-LDL for 24 h, and the expressions of CD1a, CD40, CD86, CD83, and HLA-DR on DCs were analyzed by flow cytometry. 100 ng/mL LPS treatment is a positive control. (c, d) CXCR4 and CCCR7 on DCs were analyzed by flow cytometry. 100 ng/mL LPS treatment is a positive control. (e) T- cell proliferation determined by BrdU incorporation assay. After the indicated treatment, DCs at day 7 were washed and cocultured in triplicate for 5 days with autologous T cells. 10 μmol L−1 BrdU was present in the last 16 h. Proliferative response was evaluated as OD value. Results represent the of seven-eight experiments. , compared with control cells; , compared with ox-LDL treated cells.

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