TREM-1 Modulates Dendritic Cells Maturation and Dendritic Cell-Mediated T-Cell Activation Induced by ox-LDL

Figure 9

TREM-1 was involved in DC maturation, proinflammatory cytokine production, and T-cell proliferation in plaque patients induced by ox-LDL. DCs were generated from peripheral blood monocytes of patients who underwent carotid endarterectomy. The same protocol is used as that for DCs of normal donors. Briefly, DCs at day 6 were treated with 50 μg mL⁻¹ ox-LDL for 24 h. (a) The expressions of CD1a, CD40, CD86, CD83, and HLA‐DR on day 7 DCs were analyzed by flow cytometry. (b) T‐cell proliferation determined by BrdU incorporation assay. After the indicated treatment, DCs were washed and cocultured in triplicate for 5 days with autologous T cells. 10 μmol L⁻¹ BrdU was present in the last 16 h. Proliferative response was evaluated as OD value. (c) Cytokines production were tested by ELISA. Results represent the mean ± SD of seven-eight experiments. , compared with control cells; , compared with ox-LDL-treated cells.

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