CCAAT/Enhancer-Binding Protein Delta Regulates Glioblastoma Survival through Catalase-Mediated Hydrogen Peroxide Clearance

Figure 2

Relative CAT expression is higher and associated with CEBPD expression in GBM tissues. (a) Cells from U373MG stable clones were harvested and subjected to microarray analysis. Clustering of microarray data from RNA of cells as shown identified significant gene expression clusters resulting from CEBPD downregulation (upper panel). The IPA software program was applied on 4304 potential CEBPD-regulated genes to identify top 5 scoring canonical activation or inhibition pathways (lower panel). (b) Analyses of CAT mRNA expression from GEO databases in GBM and normal brain tissues. (c) The mRNA expression levels of CEBPD and CAT are higher in human GBM tissues according to TCGA_GBM database. (d) The mRNA expression of CAT positively correlates with CEBPD in GBM tissues according to TCGA_GBM database. The summary data are presented as the ; Student’s -test; . shLuc: shRNA for luciferase; shB7: shRNA for CEBPD.

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