CCAAT/Enhancer-Binding Protein Delta Regulates Glioblastoma Survival through Catalase-Mediated Hydrogen Peroxide Clearance

Figure 4

CEBPD knockdown elevates H₂O₂ accumulation and impairs mitochondrial function. (a) U373MG or T98G cells were transiently transfected with control siRNA or CEBPD siRNA. After 48 h, cells were treated with or without H₂O₂ for 6 h and then harvested for the measurement of H₂O₂ levels. (b, c) The mitochondrial activities of (b) CEBPD stable knockdown clone T98G or (c) CEBPD transient knockdown U373MG cells are shown as the oxygen consumption rate (OCR) determined by the Seahorse XF Mito stress test. Bars represent the from three independent experiments. Differences among groups were determined with one-way or two-way ANOVA followed by Tukey’s multiple comparison test. , , and . ns: no significant; shLuc: shRNA for luciferase; shB7, shC7: shRNAs for CEBPD; siNeg: siRNA for negative control; si2895, si2896: siRNAs for CEBPD.

(a)

(b)

(c)