Mycophenolic Acid Induces the Intestinal Epithelial Barrier Damage through Mitochondrial ROS

Figure 7

Effects of MPA on the release of Cytochrome C and Bax protein expression. Cytoplasmic (Cyto) and mitochondrial (Mito) fractions were prepared from Caco-2 cells treated with different concentrations of MPA for 24 h to analyze Cytochrome C and Bax protein expression (a). After treatment of Caco-2 cells with 10 μM and 80 μM MPA for 24 h, the distribution of Mito-tracker (red) and Cytochrome C (green) was examined by confocal microscopy (b). Data are expressed as the , ; , significantly different from the control; the bar represents 10 μm.

(a)

(b)