Mycophenolic Acid Induces the Intestinal Epithelial Barrier Damage through Mitochondrial ROS

Figure 8

Effect of mitochondria-targeted antioxidant SS-31 on MPA-induced cytotoxicity. Effect of SS-31, NAC, and DPI on mitochondrial ROS in MPA-treated Caco-2 cells detected by MitoSOX staining (a). Effect of SS-31 on intracellular ROS detected by flow cytometry (b); protein expression of occludin, ZO-1 and Bax/Bcl-2 in Caco-2 cells assessed by Western blot (c, d). Data are expressed as the , ; , significantly different from the control; ^#, ^## significantly different from the mycophenolic acid-treated cells. The bar represents 50 μm.

(a)

(b)

(c)

(d)