[Retracted] DNA Topoisomerase II-α Regulated by miR-22-5p Promotes Hepatocellular Carcinoma Invasion and Migration through the Hippo Pathway

Figure 3

TOP2A knockdown inhibited the invasion and migration of HCC cell lines. (a) The mRNA level of TOP2A in normal liver cell lines and HCC cell lines was determined by RT-qPCR. (b) The protein expression of TOP2A in normal liver cell lines and HCC cell lines was determined by western blot analysis. (c) The TOP2A expression level in MHCC97L and BEL-7404 cells transfected with siRNAs targeting TOP2A was assessed by RT-qPCR. (d) The TOP2A expression level in MHCC97L and BEL-7404 cells transfected with siRNAs targeting TOP2A was determined by western blot analysis. (e) Transwell invasion assay of HCC cells transfected with si-Ctrl or si-TOP2A. The bar graphs show the number of migrating and invasive cells after 24 h. (f) Representative images of wound healing assays after scratch wounding of the cells described. (g) Western blot analysis was performed to detect the expression of EMT-related protein in HCC cells transfected with si-Ctrl or si-TOP2A. Data are shown as ; , , and .

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