[Retracted] DNA Topoisomerase II-α Regulated by miR-22-5p Promotes Hepatocellular Carcinoma Invasion and Migration through the Hippo Pathway

Figure 6

miR-22-5p inhibits the cancer-promoting effect of TOP2A. (a) Venn diagram shows the miRNAs predicted by the database that can interact with TOP2A mRNA. (b) qRT-PCR revealed the miRNA expression in liver cancer tissues and ADT. (c) qRT-PCR showed that the expression level of TOP2A mRNA in the HCC cell line (MHCC97L) transfected with miRNAs. (d) Western blot analysis demonstrated the TOP2A protein expression in the HCC cell line (MHCC97L and BEL-7404) transfected with miRNAs (e) RNA levels of miR-22-5p, GAPDH, and U6 in the nuclear and cytoplasmic fractions of MHCC97L cells. (f) Wound healing assays of HCC cells (MHCC97L and BEL-7404) transfected with miR-Ctrl or miR-22-5p mimics. (g) Transwell invasion assay of HCC cells (MHCC97L and BEL-7404) transfected with miR-Ctrl or miR-22-5p mimics. The bar graphs show the number of migrating and invasive cells after 24 h. (h) Western blotting was utilized for detection E-cad and Hippo pathway-associated protein expression in HCC cells (MHCC97L and BEL-7404) transfected with miR-Ctrl or miR-22-5p mimics. (i) Dual-luciferase reporter assay to detect the activity of LUC-TOP2A-wt or LUC-TOP2A-mutant in MHCC97L cells after overexpression of miR-22-5p. The predicted binding site of miR-22-5p in TOP2A. The mut sequence contains a 7-base mutation in the miR-22-5p target seed region. The wild-type and a mutated type of binding site between miR-22-5p and TOP2A.Data are shown as ; , , and .

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