Effect of Shenfu Injection on Differentiation of Bone Marrow Mesenchymal Stem Cells into Pacemaker-Like Cells and Improvement of Pacing Function of Sinoatrial Node

Figure 2

Shenfu injection (SFI) promotes the differentiation of BMSCs into pacemaker-like cells. (a) Cell Counting Kit-8 was utilized to detect the effect of SFI on the viability of BMSCs. , , vs. 0 μL/mL. (b) BMSCs were clamped at -70 mV and stimulated with hyperpolarizing voltages ranging from -70 to -150 mV for 3 s in 10 mV steps to record cellular currents. (c) Whole-cell patch-clamp electrophysiology was conducted and used for the purpose of recording the current on BMSCs with different treatments. (d) SFI treatment could induce the expression of HCN4 channel in BMSCs. vs. LV-NC, ^# vs. SFI-H under ZD7288. Data represented , . SFI-L: Shenfu injection-low dose (10 μL/mL); SFI-M: Shenfu injection-medium dose (20 μL/mL); SFI-H: Shenfu injection-high dose (40 μL/mL).

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