M1 Microglia Induced Neuronal Injury on Ischemic Stroke via Mitochondrial Crosstalk between Microglia and Neurons

Figure 2

Microglia was activated to M1 type in vivo and in vitro after IS. Normal BV2 cells were regarded as the control group. (a) The activation of M1 type microglia was detected by determining the expression of iNOS (M1 type microglia marker) in OGD/R-stimulated BV2 cells (). Results are presented in a form of , and indicates . (b) Immunofluorescence image of expressing of iNOS in OGD/R-stimulated BV2 cells. Scale bar: 100 μm. (c) IL-1β, IL-2, and TNF-α levels in OGD/R-stimulated BV2 cells based on western blotting (). Results are displayed in a form of ; and . (d) Schematic diagram showing brain slice as a comparison between infarct side and noninfarct side. (e) WB assay regarding iNOS protein level in infarct region and noninfarct region from brain tissues (). Results are displayed in a form of ; . (f) Immunofluorescence and quantitative assays of iNOS in infarct region and noninfarct region from brain tissues (). The relative number of M1 microglia was determined by calculating the ratio of the number of iNOS-positive cells in groups to the number of iNOS-positive cells in noninfarct region from brain tissues. Scale bar: 50 μm. Results are displayed in a form of and .

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