M1 Microglia Induced Neuronal Injury on Ischemic Stroke via Mitochondrial Crosstalk between Microglia and Neurons

Figure 4

Functional changes of released mitochondria secreted by activated microglia (M1). (a) Morphological characterization of released mitochondria isolated from culture medium of M0 microglia (Mito/M0-BV2) and M1 microglia (Mito/M1-BV2). Scale bar: 1.0 μm. (b) Levels of proteins associated with mitochondrial fission in Mito/M0-BV2 and Mito/M1-BV2 based on the western blotting (). Results are displayed in a form of ; , , and . (c) The expression of associated fusion protein, TOM20, and Cyto C in Mito/M0-BV2 and Mito/M1-BV2 (). Results are displayed in a form of , , and . (d) After OGD/R for 24 hours, function of Mito/M0-BV2 and Mito/M1-BV2 was detected by checking ATP (), mitochondria membrane potential (), and ROS (). The relative ratio of extracellular ATP in secreted mitochondria from BV2 cells was determined by calculating the ratio of level of ATP in Mito/M0-BV2 and Mito/M1-BV2 to level of ATP in Mito/M0-BV2. Results are displayed in a form of ; and .

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