M1 Microglia Induced Neuronal Injury on Ischemic Stroke via Mitochondrial Crosstalk between Microglia and Neurons

Figure 5

Microglial mitochondria can transfer to neurons and fuse with neuronal mitochondria for affecting neuronal mitochondrial function. (a) Mitochondria were obtained from the culture medium of M1 BV2 cells (Mito/M1-BV2) and OGD/R-stimulated SH-SY5Y cells (Mito/OGD/R/SH-SY5Y). Confocal image of fusion of Mito/M1-BV2 (red) and Mito/OGD/R/SH-SY5Y (green). Scale bar: 100 μm. (b) Confocal image of fusion of Mito/M1-BV2 (green) and intracellular mitochondria in SH-SY5Y cells (Mito in SH-SY5Y) (red). Scale bar: 100 μm. (c) Detection of mitochondrial ATP (), JC-1(), and ROS () in intracellular mitochondria of OGD/R-stimulated SH-SY5Y cells incubated with Mito/M1-BV2 and Mito/M0-BV2 for 24 hours. Results are displayed in a form of . and . (d) Confocal images of Mito Tracker Red-labeled intracellular mitochondria of OGD/R-stimulated SH-SY5Y cells (Mito in SH-SY5Y) treated with Mito/M1-BV2 and Mito/M0-BV2 at a scale of 100 μm. (e) TEM of mitochondrial structure in intracellular mitochondria of OGD/R-stimulated SH-SY5Y cells (Mito in SH-SY5Y) treated with Mito/M1-BV2 and Mito/M0-BV2. Scale bar: 1.0 μm. (f) Western blot analysis of TOM 20 and cytochrome c in intracellular mitochondria of OGD/R-stimulated SH-SY5Y cells (Mito in SH-SY5Y) treated with Mito/M1-BV2 and Mito/M0-BV2 (). Results are displayed in a form of ; and .

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