M1 Microglia Induced Neuronal Injury on Ischemic Stroke via Mitochondrial Crosstalk between Microglia and Neurons

Figure 6

Released mitochondria secreted by activated microglia (M1) can aggravate the damage of OGD/R-stimulated neurons. (a) Experimental chart. (b) Cell viability after coincubation of OGD/R-stimulated SH-SY5Y cells with Mito/M1-BV2 and Mito/M0-BV2 for 24 hours (). Results are displayed in a form of ; and . (c) Imaging observation and quantitative findings of apoptotic cells after coincubation of OGD/R-stimulated SH-SY5Y cells treated with Mito/M1-BV2 and Mito/M0-BV2 for 24 hours (). Apoptosis Index was determined by calculating the ratio of number of TUNEL positive cells to total Number of nuclei. Scale bar: 100 μm. Results are displayed in a form of ; . (d) Apoptotic protein levels, including BAX, BCL-2, and caspase 3 in OGD/R-stimulated SH-SY5Y cells treated with Mito/M1-BV2 and Mito/M0-BV2 based on the western blotting (). Results are displayed in a form of . , , and .

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