M1 Microglia Induced Neuronal Injury on Ischemic Stroke via Mitochondrial Crosstalk between Microglia and Neurons

Figure 7

Neurological effects of Mito/M1-BV2 and Mito/M0-BV2 on tMCAO rats. (a) After Mito/M1-BV2 was labeled red and injected into the ischemic cortex of rats, the immunofluorescent staining revealed colocalization of Mito Tracker Red-labeled microglial mitochondria with neurons labeled with anti-NeuN (green). Scale bar: 20 μm. (b) Cerebral sections () were stained using 2,3,5-triphenyltetrachloroammonium (TTC). After ischemia for 2 h and reperfusion for 6 h, the infarct volume was calculated. In addition, Ludmila belayev () and Zea-longa neurological scores () were determined. The data indicate , , and . (c) The immunofluorescent staining of NeuN-positive cells (green) in ischemic region of tMCAO rats treated with Mito/M0-BV2 and Mito/M1-BV2. NeuN antibody was utilized for neuron staining in ischemic cerebral tissues of tMCAO rats (). DAPI (blue) served as the nuclear marker. NeuN-positive neuronal counts were quantified. The relative neuron number was determined by calculating the ratio of the number of NeuN-positive cells in groups to the number of NeuN-positive cells in the sham group. Scale bar: 100 μm. Data presented are . . (d) TEM images of mitochondria in ischemic cerebral tissues of tMCAO rats. Scale bar: 0.5 μm.

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