[Retracted] Carveol Promotes Nrf2 Contribution in Depressive Disorders through an Anti-inflammatory Mechanism

Figure 5

Effects of carveol on LPS-induced downregulation of Nrf2 and HO-1. (a) RT-PCR analysis of Nrf2 in the cortex. (b) Nrf2 protein level was measured by ELISA in the cortex and hippocampus. (c) Immunohistochemistry analysis of Nrf2 in cortex and hippocampus. Data are expressed as . and are compared to the saline group; ## and # indicate a significant difference compared to the LPS group, while ^† is compared to ATRA+LPS. A thick black arrow indicates nuclear localization of Nrf2 (d) RT-PCR analysis of HO-1 in the cortex. (e) HO-1 protein level was measured by ELISA in the cortex and hippocampus. (f) Immunohistochemistry analysis of HO-1 in cortex and hippocampus. Data are expressed as . and are compared to the saline group; ## and # indicate a significant difference compared to the LPS group, while † is compared to ATRA+LPS. The saline, LPS, CAR+LPS, and FLX + LPS groups were those studied in the first cohort (/group), while the ATRA+LPS, CAR+ATRA+LPS, and ATRA+LPS+ FLX groups were from the second cohort (/group). CAR 20: carveol (20 mg/kg); LPS: lipopolysaccharide; ATRA: all-trans retinoic acid; FLX: fluoxetine; Nrf2: nuclear factor E2-related factor; HO-1: heme oxygenase 1.

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