Quercetin Protects Ethanol-Induced Hepatocyte Pyroptosis via Scavenging Mitochondrial ROS and Promoting PGC-1α-Regulated Mitochondrial Homeostasis in L02 Cells

Table 1

Information about experimental materials.

Raw materials	Biotechnology Co., Ltd	ID
Quercetin ()	MUST, Chengdu, China	117395
Cell Counting Kit-8	G-clone, Beijing, China	CC1410-500
Ethanol and other organic reagents	Kelong, Guangdong, China
Total antioxidant status (TAS) ELISA kit	Elabscience, Wuhan, China	E-BC-K801
Total oxidant status (TOS) ELISA kit	Elabscience, Wuhan, China	E-BC-K802
LPO ELISA kit	MeiMian, Jiangsu, China	MM-1378H1
Oil red storage solution	Solar, Wuhan, China	G1015
Nile Red staining solution	Solar, Wuhan, China	N8440
JC-1 Mitochondrial Membrane Potential Assay	Solar, Wuhan, China	G1515
γh2AX antibody	Solar, Wuhan, China	GB111841
MitoSOX Red Mitochondrial Superoxide	Yeasen, Shanghai, China	40778ES50
DAPI staining solution	Yeasen, Shanghai, China	40728ES03
Mito Tracker Green	KeyGEN, Jiangsu, China	KGMP007
IL-18 antibody	Affinity, Cincinnati, OH, USA	DF6252
IL-1β antibody	Affinity, Cincinnati, OH, USA	AF5103
Caspase 1 antibody	Affinity, Cincinnati, OH, USA	AF5418
Cleaved-caspase 1 (Ala317), p10 antibody	Affinity, Cincinnati, OH, USA	AF4022
NLRP3 antibody	Affinity, Cincinnati, OH, USA	DF7438
GSDMD-N-terminal antibody	Affinity, Cincinnati, OH, USA	DF12275
GAPDH antibody	Affinity, Cincinnati, OH, USA	AF0911
Cleaved-aspase3 (Asp175), p17 antibody	Affinity, Cincinnati, OH, USA	AF7022
Bcl2 antibody	Abmart, Shanghai, China	T40056
Bax antibody	Abmart, Shanghai, China	TP51006
Caspase3 antibody	Abmart, Shanghai, China	T55051
ASC/TMS1	ZEN, Chengdu, China	340097
PCG-1α	ZEN, Chengdu, China	862041
Goat Anti-Rabbit IgG(H+L) HRP secondary antibody	Multi science, Hangzhou, China	GAR007
Goat Anti-Rabbit IgG (H+L)-Cy3 secondary antibody	ABclonal, Wuhan, China	AS040
Total RNA isolation kit	Foregene, Chengdu, China	RE-03014
Animal tissue DNA isolation kit	Foregene, Chengdu, China	DE-05011
Master Premix for first-strand cDNA synthesis	Foregene, Chengdu, China	RT-01021
Real-Time PCR EasyTM-SYBR Green I	Foregene, Chengdu, China	QP-01011