Irisin Ameliorates Oxidative Stress-Induced Injury in Pancreatic Beta-Cells by Inhibiting Txnip and Inducing Stat3-Trx2 Pathway Activation

Figure 1

Irisin exerted protective effects on INS-1 cell apoptosis and dysfunction under PA treatment. (a) Cell viability analysis with Irisin treatment at different concentrations from 6 hrs to 48 hrs; the control at 0 nM ( independent experiments). (b) Cell viability analysis with PA and Irisin cotreatment for 24 hrs, PA at 0.2 mM, 0.4 mM, and 0.5 mM; Irisin at 100 nM; corresponding BSA volumes were set as controls ( independent experiments). (c) INS-1 cell apoptosis with PA and Irisin cotreatment for 24 hrs, PA at 0.2 mM; Irisin at 100 nM; Irisin NA at 100 nM; BSA was the control ( independent experiments). (d) Insulin secretion after glucose stimulation was measured by ELISA. (e) The secretory function of INS-1 cells is presented as the ratio of insulin, that is, secretion after 16.7 mM glucose treatment to that secreted after 3.3 mM glucose treatment ( independent experiments). The data are expressed as ; vs. the control; ^# vs. the PA treatment group; ^& vs. the PA and Irisin cotreatment group. BSA: bovine serum albumin; PA: palmitic acid; Irisin NA: anti-Irisin-neutralizing antibody.

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