Irisin Ameliorates Oxidative Stress-Induced Injury in Pancreatic Beta-Cells by Inhibiting Txnip and Inducing Stat3-Trx2 Pathway Activation

Figure 2

Irisin protected INS-1 cells from oxidative stress. PA at 0.2 mM; Irisin at 100 nM; Irisin NA at 100 nM; BSA set as the control. (a) Levels of cytosolic ROS were detected with a DCFH-DA probe. The DCF green signal was captured, and the MFI (mean fluorescence intensity) was calculated and is shown in histogram to quantify intracellular ROS ( independent experiments). (b) Superoxide content in INS-1 cells with PA and Irisin cotreatment is presented as the OD value at 450 nm and is shown in bar chart ( independent experiments). (c) Mitochondrial ROS levels were detected with the MitoSOX probe, and the MFI calculated was calculated and is shown in histogram to quantify the levels of mitochondrial ROS ( independent experiments). (d) Mitochondrial ROS tagged by MitoSOX were captured by fluorescence microscope (×100), and the fluorescence intensity is shown in histogram ( independent experiments). (e) MDA content in INS-1 cells in different groups ( independent experiments). The data are expressed as ; vs. the control; ^# vs. PA treatment group; ^& vs. PA and Irisin cotreatment group. BSA: bovine serum albumin; PA: palmitic acid; Irisin NA: Irisin-neutralizing antibody.

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